Crystallization of the effector-binding domains of BenM and CatM, LysR-type transcriptional regulators from Acinetobacter sp. ADP1.
Acta Crystallogr D Biol Crystallogr
; 60(Pt 1): 105-8, 2004 Jan.
Article
em En
| MEDLINE
| ID: mdl-14684899
ABSTRACT
BenM, a member of the LysR-type family of transcriptional regulators, controls genes for benzoate degradation in the Gram-negative bacterium Acinetobacter sp. strain ADP1. Recent studies show that BenM activates benABCDE expression synergistically in response to two effector ligands cis,cis-muconate (CCM) and benzoate. As an initial step in investigating the structural basis of dual effector response, the effector-binding domain of BenM (BenM-EBD) was crystallized by the microbatch-under-oil technique with conditions optimized from high-throughput screens performed by the Hauptman-Woodward Institute. Data-collection quality crystals of BenM-EBD belonged to space group P2(1)2(1)2(1), diffracted to 2.3 A and had unit-cell parameters a = 65.64, b = 66.34, c = 117.46 A. The influence of effector ligands on crystal formation was also evaluated. The presence of benzoate or CCM impaired the formation of crystals. The presence of both effectors together resulted in a dramatic decrease in the production of crystals. The effector-binding domain of CatM, a homolog of BenM, was also crystallized.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Sórbico
/
Proteínas de Bactérias
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Fatores de Transcrição
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Acinetobacter
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Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article