W3Y single mutant of rubredoxin from Pyrococcus furiosus: a preliminary time-of-flight neutron study.
Acta Crystallogr D Biol Crystallogr
; 60(Pt 1): 200-2, 2004 Jan.
Article
em En
| MEDLINE
| ID: mdl-14684930
ABSTRACT
Rubredoxin from the hyperthermophilic archaeon Pyrococcus furiosus maintains its native structure at high temperatures (373 K). In order to investigate the role of hydrogen bonding, hydration and chain dynamics in this thermostability, wavelength-resolved Laue neutron diffraction data have been collected from the W3Y single mutant (Trp3-->Tyr3) on the spallation neutron protein crystallography station (PCS) at Los Alamos Neutron Science Center. Data were measured at room temperature from nine crystal settings, each of approximately 12 h duration. The total data-measurement period was less than 5 d from a single crystal that had undergone H(2)O/D(2)O exchange. The nominal resolution of the data is 2.1 A.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rubredoxinas
/
Pyrococcus furiosus
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article