FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly.
Nat Cell Biol
; 6(2): 154-61, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14743221
ABSTRACT
Cell migration is a complex, highly regulated process that involves the continuous formation and disassembly of adhesions (adhesion turnover). Adhesion formation takes place at the leading edge of protrusions, whereas disassembly occurs both at the cell rear and at the base of protrusions. Despite the importance of these processes in migration, the mechanisms that regulate adhesion formation and disassembly remain largely unknown. Here we develop quantitative assays to measure the rate of incorporation of molecules into adhesions and the departure of these proteins from adhesions. Using these assays, we show that kinases and adaptor molecules, including focal adhesion kinase (FAK), Src, p130CAS, paxillin, extracellular signal-regulated kinase (ERK) and myosin light-chain kinase (MLCK) are critical for adhesion turnover at the cell front, a process central to migration.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Quinase de Cadeia Leve de Miosina
/
Proteínas Tirosina Quinases
/
Transdução de Sinais
/
Proteínas
/
Adesão Celular
/
Quinases da Família src
/
Proteínas Quinases Ativadas por Mitógeno
/
Proteínas do Citoesqueleto
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article