Purification and identification of a Ca(2+)-pectate binding peroxidase from Arabidopsis leaves.
Phytochemistry
; 65(3): 307-12, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14751301
A protein fraction was obtained from Arabidopsis (Arabidopsis thaliana, L.) leaf extract by affinity chromatography through a Ca(2+)-pectate/polyacrylamide gel. Further purification by preparative isoelectric focusing and SDS PAGE allowed the separation of a peroxidase that was identified as being peroxidase AtPrx34 (AtprxCb, accession number X71794) by N-terminal amino acid microsequencing. AtPrx34 belongs to a group of five Arabidopsis sequences encoding putative pectin-binding peroxidases. An expression study showed that it is expressed in root, stem, flower and leaf. It was produced by Escherichia coli and tested for its ability to bind to Ca(2+)-pectate. The identity of the amino acids involved in the interaction between the peroxidase and the Ca(2+)-pectate structure is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Proteínas de Ligação ao Cálcio
/
Pectinas
/
Arabidopsis
/
Proteínas de Arabidopsis
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article