Occurrence and linkage between secreted insecticidal toxins in natural isolates of Bacillus thuringiensis.
Curr Microbiol
; 47(6): 501-7, 2003 Dec.
Article
em En
| MEDLINE
| ID: mdl-14756535
ABSTRACT
Little is known about the occurrence and linkage between secreted insecticidal virulence factors in natural populations of Bacillus thuringiensis (Bt). We carried out a survey of 392 Bt strains isolated from various samples originating from 31 countries. The toxicity profile of the culture supernatants of these strains was determined individually against Anthonomus grandis (Coleoptera) and Spodoptera littoralis (Lepidoptera). We analyzed beta-exotoxin I production and searched for the genes encoding Vip1-2, Vip3, and Cry1I toxins in 125 of these strains. Our results showed that these insecticidal toxins were widespread in Bt but that their distribution was nonrandom, with significant linkage observed between vip3 and cry1I and between vip1-2 and beta-exotoxin I. Strains producing significant amounts of beta-exotoxin I were more frequently isolated from invertebrate samples than from dust, water, soil, or plant samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus thuringiensis
/
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Adenosina
/
Endotoxinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article