Baculovirus-mediated gene delivery into mammalian cells.
Methods Mol Biol
; 246: 355-65, 2004.
Article
em En
| MEDLINE
| ID: mdl-14970604
ABSTRACT
A relatively recent advance in the use of recombinant baculoviruses is their use for delivery of genes and genetic elements into mammalian cells. Baculovirus vectors retrofitted with mammalian gene promoters have been shown to efficiently deliver and express genes in a broad assortment of cell types. These baculovirus transductions are simple to perform, reproducible, and demonstrate no overt cell toxicity. Baculovirus-mediated gene delivery is particularly useful for repetitive or moderately high-throughput procedures such as cell-based assays, or for situations where transfection procedures are inadequate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Baculoviridae
/
Técnicas de Transferência de Genes
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article