Caveolin-1 isoform reorganization studied by image correlation spectroscopy.
Faraday Discuss
; 126: 185-95; discussion 245-54, 2004.
Article
em En
| MEDLINE
| ID: mdl-14992406
ABSTRACT
Caveolae are small, flask shaped invaginations in the cell membrane. They are thought to play a crucial role in cell signaling, endocytosis and intracellular cholesterol transport. Caveolin-1, 2 and 3 are key proteins, which are important for the formation of the invaginations on the cell surface. Caveolin-1 exists in two isoforms caveolin-1 alpha (a) and caveolin-1 beta (beta). Little is known about the difference between these two isoforms, and less in known about their role in cell signaling. Bone morphogenetic proteins IBMPs) are a subfamily of the TGF beta superfamily and their response is mediated by serine/threonine kinase receptors. Epidermal growth factor (EGF) is known to signal through tyrosine kinase receptors of the ErbB family. Here we report on the aggregation and association of caveolin-1 isoforms with these receptors and the effect of BMP and EGF activation on caveolin-1 distribution in A431 cells. Our data, obtained by application of a family of image correlation spectroscopy tools, indicate that BMP and EGF stimulation lead to a rearrangement of the caveolin-1 isoforms on the cell surface. BMP as well as EGF stimulation leads to a rearrangement of the caveolin-1 P isoform into domains enriched in the caveolin-1 alpha isoform. We further show that about 20-30% of the caveolin-1 present at the surface of the cells co-localize with the EGF and BMP receptors. Using a reporter gene assay sensitive to the activation of the BMP pathway, we show that overexpression of caveolin-1beta inhibits signaling. Our data suggest that the two isoforms of caveolin-1 play different roles on the cell surface and that caveolae are dynamic structures.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Caveolinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article