[Immunodetection of RNA-dependent RNA polymerase from turnip yellow luteovirus using monoclonal antibodies]. / Immunodetektsiia RNK-zavisimoi RNK-polimerazy virusa zheltukhi turnepsa s pomoshch'iu monokonal'nykh antitel.
Bioorg Khim
; 30(1): 25-9, 2004.
Article
em Ru
| MEDLINE
| ID: mdl-15040300
ABSTRACT
Monoclonal antibodies (MAs) to the RNA-dependent RNA polymerase from turnip yellow luteovirus (TYV) were prepared using a recombinant protein as immunogen and were shown to be directed to C-terminal part of the viral replicase. These MAs were found to interact with a 70-kDa protein found in extracts from TYV-infected plants. Our result is the first successful attempt at detecting the RNA-dependent RNA polymerase of a luteovirus in infected plant extracts. We also found that the protein is not processed further and its accumulation and content in the infected plant obey a definite dynamics during the infection. The English version of the paper Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http//www.maik.ru.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Polimerase Dependente de RNA
/
Tymovirus
/
Anticorpos Monoclonais
Idioma:
Ru
Ano de publicação:
2004
Tipo de documento:
Article