Quantitative real-time polymerase chain-reaction assay allows characterization of pneumocystis infection in immunocompetent mice.
J Infect Dis
; 189(8): 1540-4, 2004 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-15073693
ABSTRACT
Pneumocystis causes pneumonia in immunodeficient hosts but also likely causes infection in healthy hosts. To characterize infection in healthy mice, we developed and validated a real-time polymerase chain reaction assay for quantitation of Pneumocystis carinii f. sp. muris. In healthy mice exposed to Pneumocystis-infected animals, organisms were first detected at 2-3 weeks, peaked at 5-6 weeks, and were cleared by 7-9 weeks. The peak organism load in healthy animals was 2-3 logs lower than that in immunodeficient animals. This approach should facilitate studies of anti-Pneumocystis immune mechanisms in healthy hosts and provide insights into the development of Pneumocystis pneumonia in immunodeficient hosts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Infecções por Pneumocystis
/
Pneumocystis carinii
/
Pulmão
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article