Discovery and directed evolution of a glyphosate tolerance gene.
Science
; 304(5674): 1151-4, 2004 May 21.
Article
em En
| MEDLINE
| ID: mdl-15155947
ABSTRACT
The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetiltransferases
/
Plantas Geneticamente Modificadas
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Evolução Molecular Direcionada
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Embaralhamento de DNA
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Glicina
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Herbicidas
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article