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Anti-clastogenic activity of two structurally related pterocarpans purified from Bituminaria bituminosa in cultured human lymphocytes.
Maurich, T; Pistelli, L; Turchi, G.
Afiliação
  • Maurich T; Mutagenesis and Biochemistry in Somatic Cells Unit, IBF CNR, Via G. Moruzzi 1, Pisa, Italy.
Mutat Res ; 561(1-2): 75-81, 2004 Jul 11.
Article em En | MEDLINE | ID: mdl-15238232
ABSTRACT
Plant-derived isoflavones are currently receiving much attention because of their phyto-estrogenic, antioxidant, anti-mutagenic, and anti-tumor activities. In this study we have evaluated the clastogenic and anti-clastogenic activities in human lymphocytes of two structurally related pterocarpans, iso-flavonoid derivatives, termed erybraedin C and bitucarpin A, recently purified from Bituminaria bituminosa and chemically characterized. Mitomycin C (MMC) and the radio-mimetic bleomycin (BL) were used as reference clastogens. The end point studied was micronucleus formation. The results obtained in this study indicate that erybraedin C and bitucarpin A, when assayed alone, do not affect either the mitotic index or the cell-proliferation index of human lymphocytes. Interestingly, both compounds appear to be non-clastogenic in the range of concentrations used. In contrast, both substances seem to affect significantly the clastogenic effects induced by BL and MMC. A 1-h pre-exposition of the cell culture to erybraedin C was necessary to display its anti-clastogenic potential against BL, whereas bitucarpin A was inactive in this respect, with a structure-activity relationship. In contrast, the clastogenic activity of MMC was significantly reduced by both erybraedin C and bitucarpin A, using either a pre-incubation schedule or simultaneous treatment. These results suggest that the protective effects displayed by the two anti-clastogenic compounds against MMC could be due to the induction or inhibition of cellular reductive metabolic enzymes.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antimutagênicos / Pterocarpanos / Fabaceae Limite: Humans Idioma: En Ano de publicação: 2004 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antimutagênicos / Pterocarpanos / Fabaceae Limite: Humans Idioma: En Ano de publicação: 2004 Tipo de documento: Article