Covalent display of oligosaccharide arrays in microtiter plates.
J Am Chem Soc
; 126(28): 8640-1, 2004 Jul 21.
Article
em En
| MEDLINE
| ID: mdl-15250702
ABSTRACT
A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 muM).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Técnicas de Química Combinatória
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article