Analytical performance of and real sample analysis with an HBV gene visual detection chip.
J Virol Methods
; 121(1): 79-84, 2004 Oct.
Article
em En
| MEDLINE
| ID: mdl-15350736
ABSTRACT
A novel hepatitis B virus (HBV) gene detection chip has been developed. The HBV-specific probes immobilized on glass slides were hybridized with polymerase chain reaction (PCR) products of different serum samples. The hybridization signal can be easily visualized upon a sandwich assay with nanoparticle amplification. The analytical performance (e.g., specificity, sensitivity, and accuracy) of this method has been evaluated. The chip-based detection method possesses a greater sensitivity and a better reproducibility than some of the conventional immunological or molecular biological methods (e.g., enzyme-linked immunosorbent assay, ELISA) and is simple, cost-effective, and highly selective.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Vírus da Hepatite B
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Técnicas de Diagnóstico Molecular
/
Genes Virais
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article