Effects of human lactoferrin on the cytoplasmic membrane of Candida albicans cells related with its candidacidal activity.
FEMS Immunol Med Microbiol
; 42(2): 181-5, 2004 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-15364102
ABSTRACT
Human lactoferrin is an innate host defence protein with antimicrobial activity that exerts a candidacidal effect in a cation concentration-dependent manner. We investigated the ability of this cationic protein (with an isoelectric point of 8.7) to permeabilize the cytoplasmic membrane of Candida albicans cells. Despite minor K(+)-release in lactoferrin-treated C. albicans cells, the killing effect was not related to an extensive membrane permeabilization, as indicated by (a) the non-release of macromolecular cytosolic constituents; (b) the non-permeabilization for extracellular propidium iodide nor for intracellular accumulated calcein; and (c) the inability to disrupt the phospholipid bilayer of 8-aminonaphthalene-1,3,6, trisulfonic acid/p-xylene-bis-pyridiniumbromide-loaded liposomes. These results suggest that lactoferrin exerts its candidacidal effect through a mechanism different from membrane permeabilization described for other cationic peptides.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida albicans
/
Membrana Celular
/
Lactoferrina
/
Antifúngicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article