Isogenic auxotrophic mutant strains in the Aspergillus fumigatus genome reference strain AF293.
Arch Microbiol
; 182(5): 346-53, 2004 Nov.
Article
em En
| MEDLINE
| ID: mdl-15365692
ABSTRACT
Aspergillus fumigatus is a ubiquitous fungus that is a frequent opportunistic pathogen in immunosuppressed patients. Because of its role as a pathogen, it is of considerable experimental interest. A set of auxotrophic isogenic strains in the A. fumigatus genome reference strain AF293 has been developed. Using molecular genetic methods, arginine and lysine auxotrophs were made by deletion of argB and lysB, respectively. Transformation of these auxotrophic strains with plasmids carrying argB or lysB, respectively, results in efficient integration at these loci. Finally, these strains are able to form stable diploids, which should further facilitate analysis of gene functions in this fungus. Furthermore, the development of this isogenic set of auxotrophic strains in the AF293 background will enable investigators to study this important opportunistic human pathogen with greater facility.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arginina
/
Aspergillus fumigatus
/
Proteínas de Bactérias
/
Transformação Genética
/
Genoma Fúngico
/
Lisina
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article