Characterization of a gene similar to BIK1 in the yeast Kluyveromyces lactis.
Yeast
; 21(13): 1067-75, 2004 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-15484289
ABSTRACT
In Saccharomyces cerevisiae, Bik1p is a microtubule plus-end-tracking protein that plays several roles in mitosis and ploidy. KlBik1p (from Kluyveromyces lactis) maintains the same structural-domain organization as does S. cerevisiae Bik1p. As part of its characterization, we constructed a stable klbik1 mutant which is sensitive to benomyl only at 14 degrees C and has a higher frequency of crescent-shaped nuclei than S. cerevisiae bik1 mutants. This phenotype is partially rescued by S. cerevisiae BIK1. Other phenotypes associated with bik1 are not present in the K. lactis mutant. By fusion to GFP we were able to show the functionality of the KlBik1p CAP-Gly domain and found that the fusion protein changes its cellular location during the cell cycle.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Kluyveromyces
/
Proteínas Fúngicas
/
Proteínas de Saccharomyces cerevisiae
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Proteínas Associadas aos Microtúbulos
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article