[Surgical procedure improvement to produce rat ischemia-reperfusion injury model with intraluminal suture].

OBJECTIVE: To describe a modified surgical approach to produce rat ischemia-reperfusion injury model with intraluminal suture. METHODS: After exposure and ligation of the common carotid artery (CCA), the left external carotid artery and pterygopalatine artery were opened in which a 3-0 nylon suture was introduced intraluminally from the distal end of the ligature of the CCA using a scalp needle. Reperfusion injury was induced by withdrawal of the suture. RESULTS: The length of the suture was about 20.0+/-1.8 mm and the success rate of model establishment was nearly 70%. The rats developed typical symptoms and pathological manifestations after the surgery. CONCLUSION: This modified surgical approach is simple for model establishment and does not require special microsurgical skills to ensure the high success rate.