Activity profile of dust mite allergen extract using substrate libraries and functional proteomic microarrays.
Chem Biol
; 11(10): 1361-72, 2004 Oct.
Article
em En
| MEDLINE
| ID: mdl-15489163
ABSTRACT
Enzymatic activity in the fecal droppings from the house dust mite has been postulated to contribute to the elicited allergic response. Screening dust mite extracts through 137,180 tetrapeptide fluorogenic substrates allowed for the characterization of proteolytic substrate specificity from the potential cysteine and serine proteases in the extract. The extract was further screened against a 4000 member peptide nucleic acid (PNA) encoded inhibitor library designed to target cysteine proteases using microarray detection. Affinity chromatography coupled with mass spectrometry identified Der p 1 as one of the proteases targeted by the PNA inhibitors in the dust mite lysate. A phenotypic readout of Der p 1 function in allergy progression was demonstrated by the inhibition of CD25 cleavage from T cells by dust mite extract that had been treated with the Der p 1 inhibitor identified from the PNA-encoded inhibitor library.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biblioteca de Peptídeos
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Antígenos de Dermatophagoides
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article