Suppression of matrix metalloproteinase production from nasal fibroblasts by fluticasone propionate in vitro.
Acta Otolaryngol
; 124(8): 964-9, 2004 Oct.
Article
em En
| MEDLINE
| ID: mdl-15513534
ABSTRACT
OBJECTIVE:
To examine the influence of fluticasone propionate (FP) on matrix metalloproteinase (MMP) production from nasal polyp fibroblasts in vitro. MATERIAL ANDMETHODS:
Fibroblasts derived from five nasal polyps were stimulated with tumor necrosis factor (TNF)-alpha in the presence of various concentrations of FP. The influence of FP on MMP production was assessed by examining the levels of MMP-2 and -9 in culture supernatants using ELISA. We also examined the influence of FP on MMP mRNA expression using reverse transcriptase polymerase chain reaction.RESULTS:
The addition of FP caused significant suppression of MMP-2 and -9 production from nasal polyp fibroblasts in response to TNF-alpha stimulation. MMP mRNA expression was also suppressed by the addition of FP to cell cultures. The minimum concentration of the agent required to cause suppression was 10(-5) M.CONCLUSION:
These results suggest that the inhibitory action of FP on tissue remodeling may underlie the clinical efficacy of corticosteroids in nasal polyposis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pólipos Nasais
/
Metaloproteinases da Matriz
/
Fibroblastos
/
Androstadienos
/
Anti-Inflamatórios
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article