X-rays affect the expression of genes involved in angiogenesis.
Anticancer Res
; 24(5A): 2941-5, 2004.
Article
em En
| MEDLINE
| ID: mdl-15517900
ABSTRACT
BACKGROUND:
We have previously shown, using the chicken embryo chorioallantoic membrane (CAM) model of in vivo angiogenesis, that X-rays act on the extracellular matrix and enhance normal and tumor-induced angiogenesis. In the present work, we studied the effect of X-rays on the gene expression of three proteins that are important regulators of angiogenesis vascular endothelial growth factor (VEGF), heparin affin regulatory peptide (HARP) and inducible nitric oxide synthase (iNOS). MATERIALS ANDMETHODS:
An area of 1 cm2 of the CAM, restricted by a plastic ring was irradiated at room temperature. The expression of the genes was studied using RT-PCR and the amounts of the mRNAs were quantified using image analysis of the corresponding agarose gels of the RT-PCR products.RESULTS:
VEGF mRNA was decreased 6 h after irradiation. However, at later time points, VEGF expression was significantly increased compared with the nonirradiated tissue. Similarly, X-rays down-regulated both HARP and iNOS expression 6 h after irradiation and the effect was reversed at later time points, similarly to the effect of X-rays on VEGF.CONCLUSION:
These data support the notion that X-rays increase the expression of genes that favor angiogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raios X
/
Proteínas de Transporte
/
Citocinas
/
Óxido Nítrico Sintase
/
Neovascularização Fisiológica
/
Fator A de Crescimento do Endotélio Vascular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article