Sulfhydryl based cationic surfactants and the impact of polyanions on disulfide bond formation: implications for gene transfer vectors.
J Drug Target
; 12(6): 347-53, 2004 Jul.
Article
em En
| MEDLINE
| ID: mdl-15545084
ABSTRACT
Compacting plasmid DNA (pDNA) into a small size is a fundamental necessity for the efficient in vivo transfer of nucleic acids to somatic cells. An approach for accomplishing this is to condense pDNA using cationic detergents with sulfhydryl groups, near their critical micelle concentration. In this study, a model surfactant was used to study how the rate of disulfide bond formation relates to environmental factors. It was shown that the thiol detergent had the ability to form a disulfide bond when oxidized and the presence of polyanions was significantly increased. The addition of a reducing agent disrupted the disulfide bonds initially, but this was followed by disulfide bond reformation in a short time period.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Compostos de Sulfidrila
/
Tensoativos
/
Técnicas de Transferência de Genes
/
Dissulfetos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article