The role of bestrophin in airway epithelial ion transport.
FEBS Lett
; 577(3): 551-4, 2004 Nov 19.
Article
em En
| MEDLINE
| ID: mdl-15556645
ABSTRACT
The purpose of this study was to identify Cl- channels in the basolateral membrane of airway epithelial cells at the molecular level. We have focused on a new family of Cl- channels, bestrophins, which have previously been identified in retinal pigment epithelium. RT-PCR, Western blot and confocal microscopy studies revealed the presence of bestrophin in airway epithelial cells. Decreasing bestrophin expression using siRNA resulted in diminished 36Cl- flux. These studies also showed that bestrophin regulation is similar to that of native basolateral Cl- channels. The data indicate that the presence of a functional bestrophin may contribute to the basolateral cell conductance in airway epithelial cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Cloreto
/
Mucosa Respiratória
/
Proteínas do Olho
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article