The detection of lipase activity in bacteria using novel chromogenic substrates.
FEMS Microbiol Lett
; 69(3): 283-7, 1992 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-1555763
ABSTRACT
The propionate (Pro), decanoate (Dec) and laurate (Lau) esters of 5-(4-hydroxy-3,5-dimethoxyphenylmethylene)-2-thioxothiazoline++ +-3-acetic acid were assessed as substrates for lipase and esterase. On hydrolysis these substrates yield an intensely red coloured phenol which could be assayed at 505 nm. The Pro ester was an effective substrate for porcine esterase and was hydrolysed at a rate 20 times greater than the Lau and Dec esters. Conversely, Pseudomonas lipase had a high activity towards the Lau and Dec esters, especially in the presence of bovine serum albumin, but little activity towards the Pro ester. The Dec and Lau were used to detect lipolytic activity in Pseudomonas strains associated with milk spoilage. For this purpose, the substrates were absorbed onto filter paper disks, which were placed over bacterial colonies growing on agar plates; activity was indicated by bright red colouration of discs within 2 h. Escherichia coli colonies hydrolysed the Pro but not the Lau or Dec esters.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas
/
Tiazóis
/
Benzoatos
/
Compostos Cromogênicos
/
Escherichia coli
/
Esterases
/
Lipase
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article