[Applying competitive polymerase chain reaction to the detection of hepatitis B virus DNA].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 35(6): 858-9, 2004 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-15573774
ABSTRACT
OBJECTIVE:
To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples.METHODS:
A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DNA.RESULTS:
In a 20 microl C-PCR system, about 60 to 200 copies of inner control DNA could give apparent co-amplification signal band after electrophoresis on a 2% agarose gel. Five of 120 samples of clinical serum (4.2%) could not be amplified.CONCLUSION:
C-PCR has the advantage of yielding information on false negative in the HBV DNA PCR assay of clinical serum samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Reação em Cadeia da Polimerase
/
Hepatite B
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article