Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein.
Acta Crystallogr D Biol Crystallogr
; 60(Pt 12 Pt 2): 2377-9, 2004 Dec.
Article
em En
| MEDLINE
| ID: mdl-15583393
ABSTRACT
The aetiological agent of an emergent outbreak of atypical pneumonia, severe acute respiratory syndrome (SARS), is a positive-stranded RNA virus (SARS-CoV) belonging to the Coronaviridae family with a genome that differs substantially from those of other known coronaviruses. Highly conserved heptad-repeat (HR1 and HR2) regions in class I viral fusion proteins, including spike protein from SARS coronavirus, interact with each other to form a six-helix bundle, which is called a fusion core. The crystal structure of the fusion core is expected to greatly facilitate drug design. Crystals of the fusion core of SARS-CoV spike protein have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K in-house. The crystals have unit-cell parameters a = 121.2, b = 66.3, c = 70.0 A, alpha = gamma = 90, beta = 107.4 degrees and belong to space group C2. Assuming the presence of six molecules per asymmetric unit, the solvent content is estimated to be about 28%.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas de Membrana
/
Proteínas do Envelope Viral
/
Proteínas Virais de Fusão
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article