In vivo autofluorescence spectrofluorometry of central serotonin.
J Neurosci Methods
; 140(1-2): 67-73, 2004 Dec 30.
Article
em En
| MEDLINE
| ID: mdl-15589336
ABSTRACT
The autofluorescence properties of serotonin (5-HT) were investigated by light spectrofluorometry in in vitro, ex vivo and in vivo experiments. Ex vivo samples were prepared from rat brain regions containing serotonin (5-HT) i.e. cortex, striatum, hippocampus. Rats were untreated (controls) or previously submitted to chronic behavioural or pharmacological treatments known to affect endogenous 5-HT levels. Autofluorescence analysis (excitation 366 nm) on hippocampus homogenates supplied with exogenous 5-HT revealed spectral alterations attributable to changes of endogenous 5-HT levels. In vivo, real time fluorescence studies were performed via a 50 microm diameter optic fiber probe stereotaxically implanted into selected brain areas of anaesthetised rats treated with fluoxetine or 5-OH-tryptophan. All autofluorescence data were consistent with those obtained in parallel experiments performed with ex vivo or in vivo voltammetry, confirming that auto-fluorescence spectroscopy is a suitable technique for the direct assessment of fluorescent neurotransmitters. This is a reliable evidence of the in vivo application of spectroscopy together with optic fiber probe for in vivo, in situ and real time measurement of 5-HT in discrete brain areas.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Encéfalo
/
Química Encefálica
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Serotonina
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Neuroquímica
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article