[Effect of ginsenoside Rg1 on expression of p21, cyclin E and CDK2 in the process of cell senescence].
Yao Xue Xue Bao
; 39(9): 673-6, 2004 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-15606011
ABSTRACT
AIM:
To explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells.METHODS:
The cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot.RESULTS:
Pretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E.CONCLUSION:
p21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Proto-Oncogênicas p21(ras)
/
Senescência Celular
/
Ciclina E
/
Ginsenosídeos
/
Quinases relacionadas a CDC2 e CDC28
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article