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Polyphyllin D is a potent apoptosis inducer in drug-resistant HepG2 cells.
Cheung, Jenny Yuen-Nei; Ong, Rose Chik-Ying; Suen, Yick-Keung; Ooi, Vincent; Wong, Henry Nai-Ching; Mak, Thomas Chung-Wai; Fung, Kwok-Pui; Yu, Biao; Kong, Siu-Kai.
Afiliação
  • Cheung JY; Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Cancer Lett ; 217(2): 203-11, 2005 Jan 20.
Article em En | MEDLINE | ID: mdl-15617838
In a search for new anticancer agents, we identified a novel compound polyphyllin D (PD) (diosgenyl alpha-L-rhamnopyranosyl-(1-->2)-(alpha-L-arabinofuranosyl)-(1-->4)]-[beta-D-glucopyranoside) that induced DNA fragmentation and phosphatidyl-serine (PS) externalization in a hepatocellular carcinoma cell line HepG2 derivative with drug resistance (R-HepG2). PD is a saponin originally found in a tradition Chinese medicinal herb Paris polyphylla. It has been used to treat liver cancer in China for many years. We evaluated the cell-killing mechanisms of this compound in R-HepG2 and its parental cells. The mitochondrial apoptotic pathway was found to be involved in the PD-induced apoptosis because PD elicited depolarization of mitochondrial transmembrane potential (DeltaPsim), generation of H2O2, as well as release of cytochrome c and apoptosis-inducing factor in a dose- and time-dependent manner. In conclusion, we show for the first time that PD is a potent anticancer agent that can overcome drug resistance in R-HepG2 cells and elicit programmed cell death via mitochondrial dysfunction.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Carcinoma Hepatocelular / Resistencia a Medicamentos Antineoplásicos / Diosgenina Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2005 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Carcinoma Hepatocelular / Resistencia a Medicamentos Antineoplásicos / Diosgenina Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2005 Tipo de documento: Article