Stereoselective, biocatalytic reductions of alpha-chloro-beta-keto esters.
J Org Chem
; 70(1): 342-5, 2005 Jan 07.
Article
em En
| MEDLINE
| ID: mdl-15624945
ABSTRACT
Eighteen known and putative reductases from baker's yeast (Saccharomyces cerevisiae) were tested for the ability to reduce a series of alpha-chloro-beta-keto esters. In nearly all cases, it was possible to produce at least two of the four possible alpha-chloro-beta-hydroxy ester diastereomers with high optical purities. The utility of this approach was demonstrated by reducing ethyl 2-chloroacetoacetate to the corresponding syn-(2R,3S)-alcohol on a multigram scale using whole cells of an Escherichia coli strain overexpressing a single yeast reductase identified from the screening studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Saccharomyces cerevisiae
/
Oxirredutases do Álcool
/
Hidrocarbonetos Clorados
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article