[Construction of Chang Liver cDNA library and immunoscreening of ADAMS related genes].

ABSTRACT

Total RNA was isolated from Chang Liver cell. The full-length cDNA was synthesized using LD PCR by RNA 5' end switching mechanism, oligo(dT) as primer. SfiI digestion sites were introduced at both 3' and 5' end of cDNA. The cDNA was ligated with gammaTriplEX2 vector and packaged. Twenty-two positive clones were obtained by immunoscreening to the library, they were sequenced and blast in NCBI, one of them was identified a novel gene. It was submitted to GenBank and obtained accession number AY078070. It may lay down a foundation for studying on functions of ADAMs related gene.