[Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 18(3): 268-71, 2004 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-15640865
ABSTRACT
OBJECTIVE:
To investigate the anti hepatitis E virus (HEV) and HEV RNA in acute and convalescent sera of patients with NonA-E acute hepatitis.METHODS:
The serum samples were taken from 95 patients who were diagnosed as acute NonA-E hepatitis. Enzyme immunoassay (EIA) was used for detecting anti-HEV Immunoglobulin G (IgG, Genolable and Wantai EIA anti-HEV kits). RT-PCR amplification of HEV RNA was based on the open reading frame 2 region of HEV and the PCR products were sequenced.RESULTS:
Sera from 95 patients who were negative for anti-HEV in acute phase were followed up for 11-35 days to detect the anti-HEV antibody in recovery phase, 16/95 (16.84%) were positive for anti-HEV (wantai EIA anti-HEV kits). Ten (62.50%) were positive for HEV RNA in acute phase. Sequence analysis showed that 4 were HEV genotype. 6 were HEV genotype; 12/95 (12.50%) were positive for anti-HEV (Genolable EIA anti-HEV kits). Seven were positive for HEV RNA; 4 belonged to HEV genotype, 3 were HEV genotype.CONCLUSION:
It is significant and necessary to detect anti HEV antibody and HEV RNA in patients with HEV infection during acute phase and convalesent phase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Anticorpos Anti-Hepatite
/
Vírus da Hepatite E
/
Hepatite E
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article