Mechanisms controlling termination of V-J recombination at the TCRgamma locus: implications for allelic and isotypic exclusion of TCRgamma chains.
J Immunol
; 174(7): 3912-9, 2005 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-15778346
ABSTRACT
Analyses of Vgamma-Jgamma rearrangements producing the most commonly expressed TCRgamma chains in over 200 gammadelta TCR(+) thymocytes showed that assembly of TCRgamma V-region genes display properties of allelic exclusion. Moreover, introduction of functionally rearranged TCRgamma and delta transgenes results in a profound inhibition of endogenous TCRgamma rearrangements in progenitor cells. The extent of TCRgamma rearrangements in these cells is best explained by a model in which initiation of TCRgamma rearrangements at both alleles is asymmetric, occurs at different frequencies depending on the V or J segments involved, and is terminated upon production of a functional gammadelta TCR. Approximately 10% of the cells studied contained two functional TCRgamma chains involving different V and Jgamma gene segments, thus defining a certain degree of isotypic inclusion. However, these cells are isotypically excluded at the level of cell surface expression possibly due to pairing restrictions between different TCRgamma and delta chains.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T
/
Genes Codificadores da Cadeia gama de Receptores de Linfócitos T
/
Alelos
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article