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[Studies on the botanical sources and DNA molecular identification of Herba Asari based on ITS sequence].
Liu, Chun-Sheng; Bai, Gen-Ben; Yan, Yu-Ning.
Afiliação
  • Liu CS; School of Pharmacology, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi ; 30(5): 329-32, 2005 Mar.
Article em Zh | MEDLINE | ID: mdl-15806961
ABSTRACT

OBJECTIVE:

To explore the genetic basis of using three species of Asarum as Herba Asari to determine the taxonomic positions of Asarum heterotropoides and A. siebodii; and to apply DNA molecular analysis as a tool for identification of Herba Asari.

METHOD:

PCR, purification, sequence analysis were prerformed.

RESULT:

MS sequences of the three Asarum species were obtained. 3 botanical sources of Herba Asari are closely clustered together on the topology tree; one inner branch is composed of A. heterotropoides and A. sieboldii, whereas another branch contains A. sieboldii. Their ITS sequences are different.

CONCLUSION:

Three plant species of Herba Asari are closely related, and there are genetic reasons that they are used as the sources of the same medicine. The classification placement of A. sieboldii is not certain. The differences of ITS sequences of the botanical sources of Herba Asari can be used as a means of identification.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas Medicinais / DNA de Plantas / DNA Espaçador Ribossômico / Asarum Tipo de estudo: Diagnostic_studies Idioma: Zh Ano de publicação: 2005 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas Medicinais / DNA de Plantas / DNA Espaçador Ribossômico / Asarum Tipo de estudo: Diagnostic_studies Idioma: Zh Ano de publicação: 2005 Tipo de documento: Article