Cardiolipin synthase of Arabidopsis thaliana.
FEBS Lett
; 579(10): 2161-5, 2005 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-15811335
ABSTRACT
Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K(m) for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Transferases (Outros Grupos de Fosfato Substituídos)
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article