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Variable phenotypes of enterocolitis in interleukin 10-deficient mice monoassociated with two different commensal bacteria.
Kim, Sandra C; Tonkonogy, Susan L; Albright, Carol A; Tsang, Julia; Balish, Edward J; Braun, Jonathon; Huycke, Mark M; Sartor, R Balfour.
Afiliação
  • Kim SC; Center for Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, NC 27599-7032, USA.
Gastroenterology ; 128(4): 891-906, 2005 Apr.
Article em En | MEDLINE | ID: mdl-15825073
BACKGROUND & AIMS: To explore the hypothesis that selective immune responses to distinct components of the intestinal microflora induce intestinal inflammation, we characterized disease kinetics and bacterial antigen-specific T-cell responses in ex germ-free interleukin 10 -/- and wild-type control mice monoassociated with Enterococcus faecalis , Escherichia coli , or Pseudomonas fluorescens . METHODS: Colitis was measured by using blinded histological scores and spontaneous interleukin 12 secretion from colonic strip culture supernatants. Interferon gamma secretion was measured from mesenteric or caudal lymph node CD4 + T cells stimulated with bacterial lysate-pulsed antigen-presenting cells. Luminal bacterial concentrations were measured by culture and quantitative polymerase chain reaction. RESULTS: Escherichia coli induced mild cecal inflammation after 3 weeks of monoassociation in interleukin 10 -/- mice. In contrast, Enterococcus faecalis-monoassociated interleukin 10 -/- mice developed distal colitis at 10-12 weeks that was progressively more severe and associated with duodenal inflammation and obstruction by 30 weeks. Neither bacterial strain induced inflammation in wild-type mice, and germ-free and Pseudomonas fluorescens-monoassociated interleukin 10 -/- mice remained disease free. CD4 + T cells from Enterococcus faecalis- or Escherichia coli-monoassociated interleukin 10 -/- mice selectively produced higher levels of interferon gamma and interleukin 4 when stimulated with antigen-presenting cells pulsed with the bacterial species that induced disease; these immune responses preceded the onset of histological inflammation in Enterococcus faecalis -monoassociated mice. Luminal bacterial concentrations did not explain regional differences in inflammation. CONCLUSIONS: Different commensal bacterial species selectively initiate immune-mediated intestinal inflammation with distinctly different kinetics and anatomic distribution in the same host.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-10 / Infecções por Bactérias Gram-Positivas / Enterococcus faecalis / Enterocolite / Infecções por Escherichia coli Limite: Animals Idioma: En Ano de publicação: 2005 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-10 / Infecções por Bactérias Gram-Positivas / Enterococcus faecalis / Enterocolite / Infecções por Escherichia coli Limite: Animals Idioma: En Ano de publicação: 2005 Tipo de documento: Article