Macrophages overexpressing tartrate-resistant acid phosphatase show altered profile of free radical production and enhanced capacity of bacterial killing.
Biochem Biophys Res Commun
; 331(1): 120-6, 2005 May 27.
Article
em En
| MEDLINE
| ID: mdl-15845367
ABSTRACT
Activated macrophages and osteoclasts express high amounts of tartrate-resistant acid phosphatase (TRACP, acp5). TRACP has a binuclear iron center with a redox-active iron that has been shown to catalyze the formation of reactive oxygen species (ROS) by Fenton's reaction. Previous studies suggest that ROS generated by TRACP may participate in degradation of endocytosed bone matrix products in resorbing osteoclasts and degradation of foreign compounds during antigen presentation in activated macrophages. Here we have compared free radical production in macrophages of TRACP overexpressing (TRACP+) and wild-type (WT) mice. TRACP overexpression increased both ROS levels and superoxide production. Nitric oxide production was increased in activated macrophages of WT mice, but not in TRACP+ mice. Macrophages from TRACP+ mice showed increased capacity of bacterial killing. Recombinant TRACP enzyme was capable of bacterial killing in the presence of hydrogen peroxide. These results suggest that TRACP has an important biological function in immune defense system.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatase Ácida
/
Espécies Reativas de Oxigênio
/
Superóxidos
/
Isoenzimas
/
Ativação de Macrófagos
/
Macrófagos
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article