Sample preparation and mass-spectrometric characterization of crystal-derived protein samples.
Acta Crystallogr D Biol Crystallogr
; 61(Pt 5): 643-5, 2005 May.
Article
em En
| MEDLINE
| ID: mdl-15858278
ABSTRACT
Mass spectrometry is often used to ascertain the accurate mass of purified protein samples prior to crystallization screening. However, in many cases data regarding the form of the protein crystallizing can also be useful, as this may differ from the original sample. Development of a simple method for the preparation and mass spectrometry of crystal-derived protein samples is described. The method is exemplified by the determination of the phosphorylation state of protein in a crystal derived from a mixture of phosphorylated and unphosphorylated protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article