Molecularly imprinted solid-phase extraction of (-)-ephedrine from Chinese Ephedra.
J Chromatogr A
; 1070(1-2): 125-30, 2005 Apr 08.
Article
em En
| MEDLINE
| ID: mdl-15861795
ABSTRACT
Method of molecularly imprinted solid phase extraction (MISPE) of (-)-ephedrine from Chinese Ephedra has been developed in the research. The molecularly imprinted polymer (MIP) with good selectivity and affinity for (-)-ephedrine was synthesized with (-)-ephedrine as the template, methacrylic acid as the functional monomer. The washing and elution conditions in MISPE were selected and optimized for efficient analyte extraction and sample clean-up. A clean analytical HPLC base line of ephedra extract was obtained after MISPE, which indicated that the sample pre-treatment was efficient. Good recovery and precision were obtained in the assessment for the MISPE-HPLC procedure, which demonstrated it is a reliable method and can be used for the determination of (-)-ephedrine in herbal ephedra.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Ephedra
/
Efedrina
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article