An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens.
J Microbiol Methods
; 63(1): 95-8, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-15893828
ABSTRACT
A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Reação em Cadeia da Polimerase
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article