Alpha-lipoic acid increases insulin sensitivity by activating AMPK in skeletal muscle.
Biochem Biophys Res Commun
; 332(3): 885-91, 2005 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-15913551
ABSTRACT
Triglyceride accumulation in skeletal muscle contributes to insulin resistance in obesity. We recently showed that alpha-lipoic acid (ALA) reduces body weight and prevents the development of diabetes in diabetes-prone obese rats by reducing triglyceride accumulation in non-adipose tissues. AMP-activated protein kinase (AMPK) is a major regulator of cellular energy metabolism. We examined whether ALA lowers triglyceride accumulation in skeletal muscle by activating AMPK. Alpha2-AMPK activity was decreased in obese rats compared to control rats. Administration of ALA to obese rats increased insulin-stimulated glucose disposal in whole body and in skeletal muscle. ALA also increased fatty acid oxidation and activated AMPK in skeletal muscle. Adenovirus-mediated administration of dominant negative AMPK into skeletal muscle prevented the ALA-induced increases in fatty acid oxidation and insulin-stimulated glucose uptake. These results suggest that ALA-induced improvement of insulin sensitivity is mediated by activation of AMPK and reduced triglyceride accumulation in skeletal muscle.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência à Insulina
/
Ácido Tióctico
/
Proteínas Serina-Treonina Quinases
/
Músculo Esquelético
/
Complexos Multienzimáticos
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article