1-ethynylpyrene-modified guanine and cytosine as optical labels for DNA hybridization.

Wagner, Clemens; Rist, Manuela; Mayer-Enthart, Elke; Wagenknecht, Hans-Achim

Wagner, Clemens; Rist, Manuela; Mayer-Enthart, Elke; Wagenknecht, Hans-Achim.

Afiliação

Wagner C; Technical University of Munich, Chemistry Department, Lichtenbergstr. 4, D-85747 Garching, Germany.

Org Biomol Chem ; 3(11): 2062-3, 2005 Jun 07.

Article em En | MEDLINE | ID: mdl-15917887

ABSTRACT

ABSTRACT

1-ethynylpyrene shows remarkable absorption changes upon DNA hybridization when it is covalently attached to the 8-position of guanine. An absorption band at approximately 420 nm is only present in the duplex, exhibits thermal melting behaviour and provides the basis for a molecular beacon together with 1-ethynylpyrene-modified cytosine.

Assuntos

Citosina/química; DNA/química; Guanina/química; Conformação de Ácido Nucleico; Pirenos/química; Sequência de Bases; Espectrometria de Fluorescência; Espectrofotometria Ultravioleta

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pirenos / DNA / Citosina / Guanina / Conformação de Ácido Nucleico Idioma: En Ano de publicação: 2005 Tipo de documento: Article

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