Multiplexed serum measurement of IgG, IgA, IgM, and IgE antibody responses to therapeutic biologicals.
Immunol Invest
; 34(2): 199-213, 2005.
Article
em En
| MEDLINE
| ID: mdl-15921159
ABSTRACT
Analytical methods characterizing the immunogenicity of therapeutic proteins are useful for monitoring, characterizing and predicting reactions to biopharmaceuticals. A multiplexed assay capable of isotyping the specific IgG, IgA, IgM and IgE (IgGAME) antibody responses against a biotherapeutic was demonstrated in a hyper-immunized cynomolgus monkey, over a 15-month period. The quantitative range of the antibody measurements was determined to be 15 ng/ml to 50 ng/ml in 10% serum. By the use of any biotinylated or fluorescently tagged therapeutic as a detector, this multiplexed isotyping assay can be broadly applied to human and non-human primate IgG, IgA, IgM and IgE immunogenicity studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Isotipos de Imunoglobulinas
/
Testes Sorológicos
/
Imunização
/
Macaca fascicularis
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article