[Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos].
Sheng Wu Gong Cheng Xue Bao
; 19(1): 30-4, 2003 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-15969032
ABSTRACT
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Desenvolvimento Embrionário
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article