[Analysis of sequence-tagged site in bcr and abl genes by DNA pooling and dHPLC].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 13(3): 468-71, 2005 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-15972144
ABSTRACT
To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing. The results showed that the polymorphism sites were detected in 4 out of the 9 STS fragments and there were 3 bases different from the reference sequence found in 3 fragments. In conclusion, the novel SNP in U07000 fragment shows significantly different frequencies between CML and controled people.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Mielogênica Crônica BCR-ABL Positiva
/
Genes abl
/
Cromatografia Líquida de Alta Pressão
/
Sitios de Sequências Rotuladas
/
Proteínas Proto-Oncogênicas c-bcr
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article