P-selectin expression, neutrophil infiltration, and histologic injury in neonates with necrotizing enterocolitis.
J Pediatr Surg
; 40(6): 942-7; discussion 947-8, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15991175
ABSTRACT
BACKGROUND/PURPOSE:
P-selectin promotes adherence of leukocytes to the endothelium in inflammatory processes. The aim of this study was to investigate the expression of P-selectin and its role in the development of inflammation in neonates with necrotizing enterocolitis (NEC).METHODS:
Twenty-nine intestinal specimens from 13 neonates with NEC and 7 control neonates with congenital gastrointestinal abnormalities were studied. Histologic damage, immunohistochemical expression of P-selectin, and polymorphonuclear cell infiltrate were graded blindly. Mann-Whitney U and Spearman rank tests were used to compare grades.RESULTS:
Expression of P-selectin was increased in NEC compared with controls in both medium-sized vessels (P = .03) and in the microcirculation (P = .03). P-selectin expression on medium-sized vessels correlated with the degree of histologic injury (P = .02, r = 0.425). P-selectin expression was greatest in areas of active inflammation but markedly lower in necrotic areas. The degree of polymorphonuclear cell infiltration strongly correlated with P-selectin expression on both medium-sized vessels (P = .004, r = 0.513) and the microcirculation (P = .001, r = 0.578).CONCLUSIONS:
Expression of P-selectin is increased in medium-sized vessels and in the microcirculation in intestinal specimens of neonates with NEC compared with neonatal controls. Expression of P-selectin is associated with the recruitment of polymorphonuclear cells and the severity of histologic injury, although P-selectin expression is lost in necrotic tissue.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Selectina-P
/
Enterocolite Necrosante
/
Infiltração de Neutrófilos
/
Inflamação
/
Mucosa Intestinal
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Infant
/
Newborn
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article