Quantification of gene expression in mouse and human renal proximal tubules.
Methods Mol Biol
; 293: 209-19, 2005.
Article
em En
| MEDLINE
| ID: mdl-16028421
ABSTRACT
The kidney consists of many functional modules called nephrons. Each nephron has a tubular structure made up of several structurally and functionally distinct segments. The analysis of individual segments requires the use of microdissection techniques. We describe protocols that have been used to successfully isolate messenger RNA from proximal tubules of both freshly prepared and archival samples using laser capture microdissection and laser-manipulated microdissection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Microdissecção
/
Túbulos Renais Proximais
/
Lasers
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article