Laser capture microdissection and PCR for analysis of human papilloma virus infection.
Methods Mol Biol
; 293: 295-300, 2005.
Article
em En
| MEDLINE
| ID: mdl-16028428
ABSTRACT
Human papilloma virus (HPV) infection is considered one of the main factors involved in the pathogenesis of endocervical adenocarcinoma. However, the cellular location of HPV in this type of tumor is controversial. We have developed a method to determine the presence of HPV type 16 in endocervical cancer cells using laser capture microdissection followed by DNA extraction and qualitative polymerase chain reaction. Our results show that HPV type 16 is present in endocervical adenocarcinoma cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Adenocarcinoma
/
Neoplasias do Colo do Útero
/
Reação em Cadeia da Polimerase
/
Infecções por Papillomavirus
/
Microdissecção
/
Lasers
Tipo de estudo:
Qualitative_research
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article