Epithelial polarity and cell separation in the neoplastic l(1)dlg-1 mutant of Drosophila.
Mech Dev
; 37(1-2): 43-56, 1992 Mar.
Article
em En
| MEDLINE
| ID: mdl-1606019
ABSTRACT
Lethal (1) discs-large-1 [l(1)dlg-1] is a non-epithelial overgrowth or neoplastic mutant of Drosophila, which results in tumor-like imaginal discs and enlarged larvae that never pupariate. In an ultrastructural analysis we found that the wing discs develop convoluted monolayers of epithelial cells characterized by well-defined apical-basal polarity and that these layered cells secrete large amounts of basement membrane material. Immuno-EM indicates that Drosophila laminin and collagen are components of this matrix. Late in development clusters or 'rosettes' of separated cells lacking cell-cell junctions and apical-basal polarity form. In in vitro culture experiments l(1)dlg-1 wing discs did not respond to a pulse of exogenous ecdysone by secreting cuticle or losing basement membrane as normal discs do. Our observations are consistent with the hypothesis that cell-cell interaction and communication is required for termination of disc cell proliferation, which must occur prior to a cellular response to ecdysone.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Asas de Animais
/
Polaridade Celular
/
Proteínas de Drosophila
/
Proteínas Supressoras de Tumor
/
Drosophila melanogaster
/
Genes Letais
/
Hormônios de Inseto
/
Proteínas de Membrana
/
Neoplasias Experimentais
Limite:
Animals
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article