Cytosolic chloride ions stimulate Ca(2+)-induced exocytosis in melanotrophs.
FEBS Lett
; 303(2-3): 221-3, 1992 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-1607022
ABSTRACT
We used the whole-cell patch-clamp technique to study the secretory activity of single cells by monitoring changes in membrane capacitance [Neher, E. and Marty, A. (1982) Proc. Natl. Acad. Sci. USA 79, 523-535] in anterior pituitary cells. Unexpectedly we have observed that increasing intracellular chloride ions stimulate Ca(2+)-induced exocytosis in a dose-dependent fashion (Kd = 12 mM). These results demonstrate a role of cytosolic chloride ions in the regulation of exocytotic secretion in anterior pituitary cells. It is suggest that chloride channels, in addition to playing a part in regulating membrane electrical activity [Korn, S.J., Bolden, A. and Horn, A. (1991) J. Physiol. 439, 423-437; Penner, R., Matthews, G. and Horn, A. (1988) Nature 334, 499-504] and cytosolic pH [Kaila, K. and Voipio, J. (1987) Nature 330, 163-165], are also involved in the modulation of cytosolic chloride concentration and thus in the control of exocytosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adeno-Hipófise
/
Cloretos
/
Cálcio
/
Citosol
/
Exocitose
Limite:
Animals
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article