Role of protein kinase G in growth and glutamine metabolism of Mycobacterium bovis BCG.
J Bacteriol
; 187(16): 5852-6, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-16077135
ABSTRACT
The survival of pathogenic mycobacteria in macrophages requires the eukaryotic enzyme-like serine/threonine protein kinase G. This kinase with unknown specificity is secreted into the cytosol of infected macrophages and inhibits phagosome-lysosome fusion. The pknG gene is the terminal gene in a putative operon containing glnH, encoding a protein potentially involved in glutamine uptake. Here, we report that the deletion of pknG did not affect either glutamine uptake or intracellular glutamine concentrations. In vitro growth of Mycobacterium bovis BCG lacking pknG was identical to that of the wild type. We conclude that in M. bovis BCG, glutamine metabolism is not regulated by protein kinase G.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases Dependentes de GMP Cíclico
/
Glutamina
/
Mycobacterium bovis
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article